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中文摘要: 2倍稀释法研究了羧甲基壳聚糖-Cu(Ⅱ)配合物（CMC-Cu）的抑菌活性，采用循环伏安法和光谱法研究了CMC-Cu配合物与鲱鱼精脱氧核糖核酸(DNA)的相互作用。结果表明：CMC-Cu配合物对枯草芽孢杆菌(B.subtilis)、金黄色葡萄球菌(St.aureus)、大肠杆菌 (E.coli)、绿脓杆菌 (P.aeruginosa)具有良好的抑制作用。CMC-Cu在0.442 V处有一灵敏的氧化峰，中心Cu2+在玻碳电极上的反应过程主要由扩散过程控制；当滴加DNA后，CMC-Cu的氧化峰电流降低，且式量电位负移。CMC-Cu的加入使DNA的吸收峰发生蓝移且有增色效应，并对DNA-中性红体系荧光具有增强效应。CMC-Cu与DNA之间的作用模式为静电作用。
中文关键词: 羧甲基壳聚糖-Cu (Ⅱ) 抑菌 鲱鱼精DNA 相互作用
Abstract:The antibacterial activity of carboxymethyl chitosan-Cu(Ⅱ) complex (CMC-Cu) was studied in vitro. The interaction between CMC-Cu and herring sperm DNA was investigated by cyclic voltammetry and spectroscopy. Results showed that CMC-Cu had good antibacterial activity against B.subtilis, St.aureus, E.coli and P.aeruginosa. An oxidation peak of CMC-Cu appeared at 0.442 V, the electrochemical reaction of the Cu2+ on the glass carbon electrode was controlled by the process of diffusion. Adding DNA into the CMC-Cu solution, the oxidation peak current decreased and the peak potential shifted to negitive. More over, the absorption intensity of DNA was strengthened and blue shifted with the increasing of CMC-Cu concentration. The fluorescence intensity of DNA-NR system was increased by CMC-Cu. The interaction mode between CMC-Cu and herring sperm DNA was electrostatic.
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基金项目:天水师范学院“青蓝”人才工程(TSA1305);中青年教师科研资助项目（TSA1307）
引用本文：
李小芳1,冯小强1,杨声2.羧甲基壳聚糖-Cu (Ⅱ)配合物的抑菌活性及其与DNA的相互作用[J].功能高分子学报,2014,27(1):.
LI Xiao fang1,FENG Xiao qiang1,YANG Sheng2.Antibacterial Activity of Carboxymethyl Chitosan-Cu (Ⅱ) Complex and Interaction with DNA[J].Journal of Functional Polymers,2014,27(1):.
李小芳1,冯小强1,杨声2.羧甲基壳聚糖-Cu (Ⅱ)配合物的抑菌活性及其与DNA的相互作用[J].功能高分子学报,2014,27(1):.
LI Xiao fang1,FENG Xiao qiang1,YANG Sheng2.Antibacterial Activity of Carboxymethyl Chitosan-Cu (Ⅱ) Complex and Interaction with DNA[J].Journal of Functional Polymers,2014,27(1):.
