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中文摘要: 以甲基丙烯酰氯、三乙胺和荧光素反应得到荧光素甲基丙烯酸酯(FMA)，将其与聚N-(2-羟丙基)甲基丙烯酰胺(HPMA)以物质的量之比1∶10混合并通过引发剂偶氮二异丁腈(AIBN)引发聚合反应，生成带有荧光探针的聚合物P(HPMA-FMA)。采用台盼蓝排染法评估了该聚合物的细胞毒性，荧光显微镜和流式细胞仪观察和检测了全反式维甲酸(ATRA)诱导HL-60细胞分化过程中，P(HPMA-FMA)被细胞吞噬后的荧光示踪效应。 结果表明：P(HPMA-FMA)的细胞毒性极低，当P(HPMA-FMA)的质量浓度为4～16 mg/mL时对细胞增殖无影响；当其质量浓度为30 μg/mL时即可满足荧光显微镜定性示踪观察和流式细胞术定量检测所需要的荧光强度。
中文关键词: N-(2-羟丙基)甲基丙烯酰胺/荧光素甲基丙烯酸酯聚合物 细胞毒性 细胞吞噬 荧光探针
Abstract:Fluorescein methyl acrylate(FMA) was prepared using the equimolar methacryloyl chloride，triethylamine and fluorescein as raw materials. A novel kind of polymer fluorescent probes P(HPMA- FMA) were prepared using FMA and N-(2-hydroxypropyl) methacrylamide(HPMA) at the molar ratio of 1∶10 ，and 2，2′-azobis(2-methylpropio nitrile)(AIBN) as initiator． The trypan blue staining was used to assessed the cytotoxicity of P(HPMA-FMA) on HL-60 cells．Fluorescence microscopy and flow cytometry were used to make a further investigation of phagocytosis of fluorescent tracing effects by P(HPMA-FMA) on human acute promyelocytic leukemia HL-60 cells．Results indicated that the P(HPMA-FMA) was less toxic to HL-60 cells and had no effect on cell proliferation with the mass concentration of 4—16 mg/mL．The fluorescence intensity could achieve the requirement to be observed by fluorescence microscopy and detected by flow cytometry only at the level of 30 μg/mL.
keywords: P(HPMA-FMA) cytotoxicity phagocytosis fluorescent probe
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基金项目:国家自然科学基金(30960464)
引用本文：
吴炎鹏1,许卫兵2,孔花青1,何苗1,丁兰1,刘国安1.P(HPMA FMA)荧光探针制备、细胞毒性评估及细胞吞噬示踪检测[J].功能高分子学报,2014,27(1):.
WU Yan peng1,XU Wei bing2,KONG Hua qing1,HE Miao1,DING Lan1,LIU Guo an1.P(HPMA-FMA) Preparation of Fluorescent Probes, Cell Toxicity Assessment and Detection of Phagocytic Tracer[J].Journal of Functional Polymers,2014,27(1):.
吴炎鹏1,许卫兵2,孔花青1,何苗1,丁兰1,刘国安1.P(HPMA FMA)荧光探针制备、细胞毒性评估及细胞吞噬示踪检测[J].功能高分子学报,2014,27(1):.
WU Yan peng1,XU Wei bing2,KONG Hua qing1,HE Miao1,DING Lan1,LIU Guo an1.P(HPMA-FMA) Preparation of Fluorescent Probes, Cell Toxicity Assessment and Detection of Phagocytic Tracer[J].Journal of Functional Polymers,2014,27(1):.
