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投稿时间：2012-07-14
投稿时间：2012-07-14
中文摘要: 以旋涂法制备的聚乳酸（PLA）、聚丙交酯己内酯（PLCL）、聚乳酸-羟基磷灰石（PLA-HA）、聚丙交酯己内酯-羟基磷灰石（PLCL-HA）膜为功能性骨组织工程支架材料引导组织再生膜，用小鼠颅骨前成骨细胞系作为组织工程种子细胞，进行了平面细胞培养，通过四唑类化合物（MTS）比色法细胞活性定量检测、二乙酸荧光素（FDA）膜染色等研究了基材对成骨细胞的黏附、增殖以及矿化的影响；通过光学显微镜和荧光显微镜进行了细胞形态的观察。结果表明：HA对细胞的黏附和增殖有积极的作用，旋涂膜上的细胞进入增殖晚期和矿化期后性质
Abstract:Two-dimensional cell culture is performed using spin coated PLA，PLCL，PLA-HA and PLCL-HA membranes as functional bone tissue engineering scaffold for guided tissue regeneration matrices and the MC3T3-E1 subclone 14 pre-osteoblasts as seed cells. The effect of different matrices on cell adhesion, proliferation and mineralization is studied using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay and FDA staining method. Cell morphology is observed with optical microscope and fluorescence microscope. Results show that HA is positive for cell adhesion and cell proliferation. The spin coated membranes show better stability in the late period of proliferation and mineralization.
keywords: spin coated polylactic acid poly(lactide-ε-caprolactone) hydroxyapatite biological evaluation
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基金项目:国家自然科学基金（30800230）；上海市科技发展基金重点纳米专项（0852nm03600)
引用本文：
袁华,李浩淼,吴国良,任天斌.旋涂法制备聚乳酸基聚酯复合膜的生物学评价[J].功能高分子学报,2012,25(3):.
YUAN Hua,LI Hao-miao,WU Guo-liang,REN Tian-bin.Biological Evaluation of Spin Coated PLA Based Polyester Composite Films[J].Journal of Functional Polymers,2012,25(3):.
袁华,李浩淼,吴国良,任天斌.旋涂法制备聚乳酸基聚酯复合膜的生物学评价[J].功能高分子学报,2012,25(3):.
YUAN Hua,LI Hao-miao,WU Guo-liang,REN Tian-bin.Biological Evaluation of Spin Coated PLA Based Polyester Composite Films[J].Journal of Functional Polymers,2012,25(3):.
