Abstract:The photosensitive monomer cinnamic acid (CA) was introduced into hyaluronic acid (HA) to prepare amphiphilic HA-CA. HA-CA colloidal particles were obtained through self-assembly in selective solvents H2O/DMSO. The structure and the modification degree of HA-CA were confirmed by ultraviolet spectroscopy (UV) and proton nuclear magnetic resonance (1H-NMR). The self-assembly behavior of HA-CA were investigated. The size and morphology of the HA-CA colloidal particles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and Zeta potential analyzers. The HA-CA colloidal particles were used as particulate emulsifier to stabilize oil-water interface and the emulsification performance of HA-CA colloidal particles in different condition such as pH value, salt concentration and the oil type were investigated. The morphology of colloidal particles in oil-water interface was observed by styrene oil phase solidification method. The cell compatibility of colloidal particles and emulsions were also studied. Results showed that HA-CA have absorbance in the range of UVB. HA-CA can self-assemble into spherical colloidal particles with the size of about 95 nm in selective solvent. HA-CA colloidal particles could be used as particulate emulsifier to prepare oil-in-water Pickering emulsion in the broader range of pH and various concentration of NaCl. Despite HA-CA colloidal particles cannot stabilize the oil-water interface to form an emulsion when pH is 6.47, adding a little inorganic salt can greatly improve it’s the emulsifying capacity in neutral condition. In addition, the colloidal particles as well as emulsions have great salt tolerance and cell compatibility. Moreover, HA-CA colloidal particles can also stabilize a wide range of oil-water system, which means it has a certain universality as a particle emulsifier.